Development of a Stable MGAT1− CHO Cell Line to Produce Clade C gp120 With Improved Binding to Broadly Neutralizing Antibodies

by Rachel C. Doran, Rachel C. Doran, Bin Yu, Meredith Wright, Sara M. O'Rourke, Lu Yin, Jennie M. Richardson, Gabriel Byrne, Kathryn A. Mesa, Phillip W. Berman
Published 2018-10-01

Glycan modifications to the gp120 immunogens used in the RV144 vaccine trial improve binding to broadly neutralizing antibodies.

by Rachel C Doran, Gwen P Tatsuno, Sara M O'Rourke, Bin Yu, David L Alexander, Kathryn A Mesa, Phillip W Berman
Published 2018-01-01

Robotic selection for the rapid development of stable CHO cell lines for HIV vaccine production.

by Sara M O'Rourke, Gabriel Byrne, Gwen Tatsuno, Meredith Wright, Bin Yu, Kathryn A Mesa, Rachel C Doran, David Alexander, Phillip W Berman
Published 2018-01-01

CRISPR/Cas9 gene editing for the creation of an MGAT1-deficient CHO cell line to control HIV-1 vaccine glycosylation.

by Gabriel Byrne, Sara M O'Rourke, David L Alexander, Bin Yu, Rachel C Doran, Meredith Wright, Qiushi Chen, Parastoo Azadi, Phillip W Berman
Published 2018-08-01