Article Biocatalysis Catalytic Domain Enzyme Stability Escherichia coli Gene fusion Hydro-Lyases Kinetics Molecular Dynamics Simulation Molecular dynamics simulation Nitrile hydratase Protein Subunits Pseudomonas putida Recombinant Proteins Temperature amide biocatalysis catalysis chemistry concentration (parameter) controlled study enzyme active site enzyme activity enzyme analysis enzyme mechanism enzyme purification enzyme stability enzyme structure hydrogen bond hydrolyase