Expansion of human mesenchymal stem/stromal cells on temporary liquid microcarriers

Expansion of human mesenchymal stem/stromal cells on temporary liquid microcarriers

BACKGROUND: Traditional large-scale culture systems for human mesenchymal stem/stromal cells (hMSCs) use solid microcarriers as attachment substrates. Although the use of such substrates is advantageous because of the high surface-to-volume ratio, cell harvest from the same substrates is a challenge...

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Bibliographic Details
Main Authors: Coopman, K. (Author), Hanga, M.P (Author), Hewitt, C.J (Author), Murasiewicz, H. (Author), Nienow, A.W (Author), Pacek, A.W (Author)
Format: Article
Language:English
Published: John Wiley and Sons Ltd 2021
Subjects:
Article
cell aggregation
cell differentiation
Cell membranes
cell structure
cell surface marker
Cell surface marker
clonogenesis
Coalescence
controlled study
Cytology
Droplet coalescence
Drops
Enzymatic treatments
Expansion
fluorocarbon
Fluorocarbons
High surface-to-volume ratio
human
human bone marrow-derived mesenchymal stem/stromal cells (hMSCs)
human cell
Large scale systems
Large-scale cultures
Liquids
mesenchymal stem cell
mesenchymal stroma cell
microcarrier culture
normal human
particle size
perfluorocarbon
proteinase
Proteolytic enzyme
Quality characteristic
scale-up
stem cell expansion
temporary microcarriers
Two phase systems
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