Article Cell membranes Cell surface marker Coalescence Cytology Droplet coalescence Drops Enzymatic treatments Expansion Fluorocarbons High surface-to-volume ratio Large scale systems Large-scale cultures Liquids Proteolytic enzyme Quality characteristic Two phase systems cell aggregation cell differentiation cell structure cell surface marker clonogenesis controlled study fluorocarbon human human bone marrow-derived mesenchymal stem/stromal cells (hMSCs) human cell mesenchymal stem cell mesenchymal stroma cell microcarrier culture