Studies on the optimization of the microencapsulation of lactic acid bacteria

• Main Authors: Tzu-Yun Huang, 黃子芸
• Other Authors: V. An-Erl King
• Format: Others
• Language: zh-TW
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/59248415726429344235

碩士 === 國立中興大學 === 食品暨應用生物科技學系所 === 103 === Latic acid bacteria could offer lots of health effects to humankind has been extensively studied, and Lactobacillus acidophilus is one of famous probiotics which plays an important role in dairy fermentation. By intake those fermented food contained live L. acidophilus can get many health benefits has been confirmed. In addition, Lactobacillus salivarius has gained attention in recent years as a promising probiotic species, has the potential with the characteristic of anti-Helicobacter pylori and well immune regulation. However, these two latic acid bacterias have been maintained a number of viable cells which has shown to be efficacious in human gastrointestinal tract. Therefore, this study used microencapsulation to entrap latic acid bacteria. A three-variable and three-level design method, analyzed by response surface methodology, was used to study the effects of sodium alginate concentration, pectin concentration, and CaCl2 concentration on the survival of L. acidophilus, and to determine the optimum conditions. Results showed that 1.5% sodium alginate, 1.5% pectin, and 0.3M CaCl2 combining with 1% chitosan had the highest survival rate, then evaluate the health effects of L. acidophilus miceocapsule prepared by this optimum conditions. This study also used extrusion to prepare microencapsulated Lactobacillus salivarius with different treatments, such as concentration, fermentation and adding trehalose. The purpose of using those different treatments to prepare probiotic microcapsules is to enhance its application. This would provide traditional probiotic microcapsule with more variability and applicability. The optimum conditions were determined by efficiency of L. salivarius and to evaluate its health effects. The results showed that the microcapsules CE1-2, CE1-0 and ES-2 have higher efficiency, and the microcapsule containing concentrated entrapped and surface L. salivarius fermented in MRS broth supplemented with 1% CaCl2(CE1-0) and without trehalose has the highest viability when treated with simulated digestive juices. Besides, the results of L. acidophilus miceocapsule prepared by optimum conditions and the CE1-0 L. salivarius microcapsule including β-galactosidase activity, acidification power test, and protein analysis tested with simulated digestive juices all showed significantly improved compared with free bacteria. Indicated these two microcapsules could improve the tolerance to simulated gastrointestinal conditions and maintain good viability, activity and metabolic activity.