Studies on the Chemical Constituents of Antrodia camphorate Submerged Whole Broth and its Essential Oil

• Main Authors: Pin-Chen Huang, 黃品臻
• Other Authors: Yi-Yuan Shao
• Format: Others
• Language: zh-TW
• Published: 2007
• Online Access: http://ndltd.ncl.edu.tw/handle/17038979341214467721

碩士 === 實踐大學 === 食品營養研究所 === 96 === Abstract The first part of study was on isolation and identification of the chemical constituents of Antrodia camphorata submerged whole broth. Powder of Antrodia camphorata submerged whole broth was extracted with methanol at room temperature three times (7 days each time). The extract was concentrated in vacuo to yield a residue which was suspended in water, and this phase was partitioned with ethyl acetate five times. The combined ethyl acetate layers afforded a black syrup which was separated by open column chromatography, thin layer chromatography, flash column chromatography, high performance liquid chromatography and recrystallization to afford 15 compounds. Among these compounds are classified to 5 sterols, 4 triterpenoids and 6 others. The 15 compounds are shown as follows: ST1: Ergosta-7,9(11),22E -trien-3β-ol ST2: Ergosterol peroxide ST3:β-Sitosterol ST4: Stigmasterol ST5: 3β-5α-dihydroxy-6β-methoxyergosta-7,22(E)-diene TR1: Dehydrosulphurenic acid TR2: 24-Methylene-lanost-8-en -3β-ol TR3: Eburicoic acid TR4: Dehydroeburicoic acid OT1: (2E,4E)-Hexa-2,4-dienoic acid OT2: Succinic acid monomethyl ester OT3: (S)-(+)-pantolactone OT4: Hydroquinone OT5*: 5-Methoxy-4-methyl-2,7-dioxa-bicyclo[4.1.0]hept-4-en-3-one OT6: Dimethyl 2-hydroxysuccinate * new compound The second part is analysis the material composition of easy to volatilize. Separately analysing the first part of research samples, and Antrodia camphorata ferment liquid upper strata float thing dry powder distillation essensitial oil sample, Antrodia camphorata ferment liquid upper strata float thing with liquid distillation essensitial oil sample, the Antrodia camphorata the whole broth of freezes the powder distillation essensitial oil sample, Antrodia camphorata the whole broth of freezes the powder methyl alcohol extract residue distillation essensitial oil sample, s Antrodia camphorata ferment liquid trough give vent to take off activated carbon essensitial oil sample with ether, in separately analysis by GC-MS. Antrodia camphorata ferment liquid upper strata float thing dry powder distillation essensitial oil sample main components are terpenoids and esters. The terpenoids in Antrodia camphorata the whole broth of freezes the powder distillation 4~8 hours essensitial oil sample WB-A2 evident higher than the essensitial oil sample WB-A1. Antrodia camphorata ferment liquid upper strata float thing with liquid distillation essensitial oil samples main components are esters. Synthetically, chemical compounds gamma-dodecalacton and delta-cadinene, should exist in the broth of Antrodia camphorata ferment liquid, and may exist in the Antrodia camphorata mycelium. Chemical compounds of (9Z,12Z) -ethyl octaeca-9,12-dienoate and ethyl oleate should mainly exist in Antrodia camphorata ferment liquid. In addition, the chemical compounds of nerolidol and farnesyl acetate3 are exist in Antrodia camphorata ferment liquid upper strata float thing. Chemical compound furfural should exist in the broth of Antrodia camphorata ferment liquid upper strata float thing, and may exist in the Antrodia camphorata mycelium. The prophase of train Antrodia camphorata ferment the air vent hole activated carbon with ether take off essensitial oil for the most part of composition is ketone, the train later stage essensitial oil main of compositions are terpenoids and esters. Therefore inference, Antrodia camphorata mycelium in a large to synthesize alcohols and terpenoids at ferment train of later stage. The value of the essensitial oil also improves relatively. In charge of first part of research essensitial oil sample obtained to analyse contain a lot of long chain ester type, steroids and triterpenoids. The sample EH20-ST1-AF to detect three kinds of steroids contains beta-sitosterol (ST3), stigmasterol (ST4) and 29- isofucosterol. Essensitial oil sample WMD whether Antrodia camphorata the whole broth of freezes the powder methyl alcohol extract residue distillation, the primary compound is the ester. When the cultivation on later stage of Antrodia camphorata ferment the air vent hole activated carbon with ether take off essensitial oil C-B content high fragrance serious. The metabolism result with fly away during the ferment to train, if can collect and study further, perhaps can develop the new products.