Genetic Engineering by DNA Recombineering
Recombineering inserts PCR products into DNA using homologous recombination. A pair of short homology arms (50 base pairs) on the ends of a PCR cassette target the cassette to its intended location. These homology arms can be easily introduced as 5' primer overhangs during the PCR reaction. The...
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| Format: | Article |
| Language: | English |
| Published: |
Wiley,
2021-01-19T20:30:09Z.
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| Subjects: | |
| Online Access: | Get fulltext |