Crystal Structure of Cas9 in Complex with Guide RNA and Target DNA

Crystal Structure of Cas9 in Complex with Guide RNA and Target DNA

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The CRISPR-associated endonuclease Cas9 can be targeted to specific genomic loci by single guide RNAs (sgRNAs). Here, we report the crystal structure of Streptococcus pyogenes Cas9 in complex with sgRNA and its target DNA at 2.5 Å resolution. The structure revealed a bilobed architecture composed o...

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Bibliographic Details
Main Authors: Nishimasu, Hiroshi (Author), Dohmae, Naoshi (Author), Ishitani, Ryuichiro (Author), Nureki, Osamu (Author), Ran, Fei (Contributor), Hsu, Patrick (Contributor), Konermann, Silvana M (Contributor), Shehata, Soraya I. (Contributor), Zhang, Feng (Contributor)
Other Authors: Massachusetts Institute of Technology. Department of Biological Engineering (Contributor), Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences (Contributor), McGovern Institute for Brain Research at MIT (Contributor)
Format: Article
Language:English
Published: Elsevier, 2017-12-13T15:02:26Z.
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