Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex

Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex

Systematic interrogation of gene function requires the ability to perturb gene expression in a robust and generalizable manner. Here we describe structure-guided engineering of a CRISPR-Cas9 complex to mediate efficient transcriptional activation at endogenous genomic loci. We used these engineered...

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Bibliographic Details
Main Authors: Konermann, Silvana (Contributor), Brigham, Mark D. (Contributor), Trevino, Alexandro E. (Contributor), Joung, Julia (Contributor), Barcena, Clea (Contributor), Hsu, Patrick D. (Contributor), Habib, Naomi (Author), Gootenberg, Jonathan S. (Contributor), Nishimasu, Hiroshi (Author), Nureki, Osamu (Author), Zhang, Feng (Contributor), Abudayyeh, Omar Osama (Contributor)
Other Authors: Harvard University- (Contributor), Massachusetts Institute of Technology. Department of Biological Engineering (Contributor), Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences (Contributor), McGovern Institute for Brain Research at MIT (Contributor)
Format: Article
Language:English
Published: 2016-05-23T00:51:14Z.
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