Careful selection of reference genes is required for reliable performance of RT-qPCR in human normal and cancer cell lines.

Reverse Transcription - quantitative Polymerase Chain Reaction (RT-qPCR) is a standard technique in most laboratories. The selection of reference genes is essential for data normalization and the selection of suitable reference genes remains critical. Our aim was to 1) review the literature since im...

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Bibliographic Details
Main Authors: Francis Jacob, Rea Guertler, Stephanie Naim, Sheri Nixdorf, André Fedier, Neville F Hacker, Viola Heinzelmann-Schwarz
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS ONE
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23554992/?tool=EBI