AcrIF9 tethers non-sequence specific dsDNA to the CRISPR RNA-guided surveillance complex

AcrIF9 tethers non-sequence specific dsDNA to the CRISPR RNA-guided surveillance complex

The anti-CRISPR protein IF9 (AcrIF9) specifically inhibits the type I-F CRISPR adaptive immune system. Here, the authors present the cryo-EM structure of AcrIF9 in complex with the type I-F CRISPR RNA-guided surveillance complex (Csy) and a dsDNA bound Csy-AcrIF9 structure, and find that AcrIF9 bind...

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Main Authors: Marscha Hirschi, Wang-Ting Lu, Andrew Santiago-Frangos, Royce Wilkinson, Sarah M. Golden, Alan R. Davidson, Gabriel C. Lander, Blake Wiedenheft
Format: Article
Language:English
Published: Nature Publishing Group 2020-06-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-020-16512-1
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spelling doaj-d3c21875e57643ea9c2043b059ad21af2021-06-06T11:14:32ZengNature Publishing GroupNature Communications2041-17232020-06-011111610.1038/s41467-020-16512-1AcrIF9 tethers non-sequence specific dsDNA to the CRISPR RNA-guided surveillance complexMarscha Hirschi0Wang-Ting Lu1Andrew Santiago-Frangos2Royce Wilkinson3Sarah M. Golden4Alan R. Davidson5Gabriel C. Lander6Blake Wiedenheft7Department of Integrative Structural and Computational Biology, The Scripps Research InstituteDepartment of Molecular Genetics, and Department of Biochemistry, University of TorontoDepartment of Microbiology and Immunology, Montana State UniversityDepartment of Microbiology and Immunology, Montana State UniversityDepartment of Microbiology and Immunology, Montana State UniversityDepartment of Molecular Genetics, and Department of Biochemistry, University of TorontoDepartment of Integrative Structural and Computational Biology, The Scripps Research InstituteDepartment of Microbiology and Immunology, Montana State UniversityThe anti-CRISPR protein IF9 (AcrIF9) specifically inhibits the type I-F CRISPR adaptive immune system. Here, the authors present the cryo-EM structure of AcrIF9 in complex with the type I-F CRISPR RNA-guided surveillance complex (Csy) and a dsDNA bound Csy-AcrIF9 structure, and find that AcrIF9 binding to the Csy complex triggers non-sequence specific dsDNA binding to Csy-AcrIF9, which might sequester the complex from its target DNA.https://doi.org/10.1038/s41467-020-16512-1
collection DOAJ
language English
format Article
sources DOAJ
author Marscha Hirschi
Wang-Ting Lu
Andrew Santiago-Frangos
Royce Wilkinson
Sarah M. Golden
Alan R. Davidson
Gabriel C. Lander
Blake Wiedenheft
spellingShingle Marscha Hirschi
Wang-Ting Lu
Andrew Santiago-Frangos
Royce Wilkinson
Sarah M. Golden
Alan R. Davidson
Gabriel C. Lander
Blake Wiedenheft
AcrIF9 tethers non-sequence specific dsDNA to the CRISPR RNA-guided surveillance complex
Nature Communications
author_facet Marscha Hirschi
Wang-Ting Lu
Andrew Santiago-Frangos
Royce Wilkinson
Sarah M. Golden
Alan R. Davidson
Gabriel C. Lander
Blake Wiedenheft
author_sort Marscha Hirschi
title AcrIF9 tethers non-sequence specific dsDNA to the CRISPR RNA-guided surveillance complex
title_short AcrIF9 tethers non-sequence specific dsDNA to the CRISPR RNA-guided surveillance complex
title_full AcrIF9 tethers non-sequence specific dsDNA to the CRISPR RNA-guided surveillance complex
title_fullStr AcrIF9 tethers non-sequence specific dsDNA to the CRISPR RNA-guided surveillance complex
title_full_unstemmed AcrIF9 tethers non-sequence specific dsDNA to the CRISPR RNA-guided surveillance complex
title_sort acrif9 tethers non-sequence specific dsdna to the crispr rna-guided surveillance complex
publisher Nature Publishing Group
series Nature Communications
issn 2041-1723
publishDate 2020-06-01
description The anti-CRISPR protein IF9 (AcrIF9) specifically inhibits the type I-F CRISPR adaptive immune system. Here, the authors present the cryo-EM structure of AcrIF9 in complex with the type I-F CRISPR RNA-guided surveillance complex (Csy) and a dsDNA bound Csy-AcrIF9 structure, and find that AcrIF9 binding to the Csy complex triggers non-sequence specific dsDNA binding to Csy-AcrIF9, which might sequester the complex from its target DNA.
url https://doi.org/10.1038/s41467-020-16512-1
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