A Novel HPLC-Based Method to Investigate on RNA after Fixation

A Novel HPLC-Based Method to Investigate on RNA after Fixation

RNA isolated from fixed and paraffin-embedded tissues is widely used in biomedical research and molecular pathology for diagnosis. In the present study, we have set-up a method based on high performance liquid chromatography (HPLC) to investigate the effects of different fixatives on RNA. By the app...

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Bibliographic Details
Main Authors: Paolo Fattorini, Cristina Forzato, Domenico Tierno, Eleonora De Martino, Eros Azzalini, Vincenzo Canzonieri, Giorgio Stanta, Serena Bonin
Format: Article
Language:English
Published: MDPI AG 2020-10-01
Series:International Journal of Molecular Sciences
Subjects:
RNA
fixation
nuclease digestion
nucleotide-monophosphate
HPLC
degradation
Online Access:https://www.mdpi.com/1422-0067/21/20/7540

Internet

https://www.mdpi.com/1422-0067/21/20/7540

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