Comparative production analysis of three phlebovirus nucleoproteins under denaturing or non-denaturing conditions for crystallographic studies.

Comparative production analysis of three phlebovirus nucleoproteins under denaturing or non-denaturing conditions for crystallographic studies.

Nucleoproteins (NPs) encapsidate the Phlebovirus genomic (-)RNA. Upon recombinant expression, NPs tend to form heterogeneous oligomers impeding characterization of the encapsidation process through crystallographic studies. To overcome this problem, we set up a standard protocol in which production...

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Bibliographic Details
Main Authors: Violaine Lantez, Karen Dalle, Rémi Charrel, Cécile Baronti, Bruno Canard, Bruno Coutard
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2011-01-01
Series:PLoS Neglected Tropical Diseases
Online Access:http://europepmc.org/articles/PMC3014985?pdf=render

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http://europepmc.org/articles/PMC3014985?pdf=render

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