Evaluation of RNA isolation methods for microRNA quantification in a range of clinical biofluids

Abstract Background Extracellular microRNAs (miRNAs), released from cells into biofluids, have emerged as promising biomarkers for diagnostic and prognostic purposes. Several RNA isolation methods are available for the analysis of these cell-free miRNAs by RT-qPCR. Not all methods, however, are equa...

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Bibliographic Details
Main Authors: Henk P. Roest, Jan N. M. IJzermans, Luc J. W. van der Laan
Format: Article
Language:English
Published: BMC 2021-08-01
Series:BMC Biotechnology
Subjects:
Online Access:https://doi.org/10.1186/s12896-021-00706-6