Hybridization capture using short PCR products enriches small genomes by capturing flanking sequences (CapFlank).

Hybridization capture using short PCR products enriches small genomes by capturing flanking sequences (CapFlank).

Solution hybridization capture methods utilize biotinylated oligonucleotides as baits to enrich homologous sequences from next generation sequencing (NGS) libraries. Coupled with NGS, the method generates kilo to gigabases of high confidence consensus targeted sequence. However, in many experiments,...

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Bibliographic Details
Main Authors: Kyriakos Tsangaras, Nathan Wales, Thomas Sicheritz-Pontén, Simon Rasmussen, Johan Michaux, Yasuko Ishida, Serge Morand, Marie-Louise Kampmann, M Thomas P Gilbert, Alex D Greenwood
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2014-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4183570?pdf=render

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http://europepmc.org/articles/PMC4183570?pdf=render

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