Multiscale ATUM-FIB Microscopy Enables Targeted Ultrastructural Analysis at Isotropic Resolution

Multiscale ATUM-FIB Microscopy Enables Targeted Ultrastructural Analysis at Isotropic Resolution

Summary: Volume electron microscopy enables the ultrastructural analysis of biological tissue. Currently, the techniques involving ultramicrotomy (ATUM, ssTEM) allow large fields of view but afford only limited z-resolution, whereas ion beam-milling approaches (FIB-SEM) yield isotropic voxels but ar...

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Bibliographic Details
Main Authors: Georg Kislinger, Helmut Gnägi, Martin Kerschensteiner, Mikael Simons, Thomas Misgeld, Martina Schifferer
Format: Article
Language:English
Published: Elsevier 2020-07-01
Series:iScience
Subjects:
Imaging Anatomy
Biological Sciences
Cellular Neuroscience
Experimental Systems for Structural Biology
Online Access:http://www.sciencedirect.com/science/article/pii/S2589004220304776

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http://www.sciencedirect.com/science/article/pii/S2589004220304776

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