Multiplexed capture of spatial configuration and temporal dynamics of locus-specific 3D chromatin by biotinylated dCas9

Multiplexed capture of spatial configuration and temporal dynamics of locus-specific 3D chromatin by biotinylated dCas9

Abstract The spatiotemporal control of 3D genome is fundamental for gene regulation, yet it remains challenging to profile high-resolution chromatin structure at cis-regulatory elements (CREs). Using C-terminally biotinylated dCas9, endogenous biotin ligases, and pooled sgRNAs, we describe the dCas9...

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Bibliographic Details
Main Authors: Xin Liu, Yong Chen, Yuannyu Zhang, Yuxuan Liu, Nan Liu, Giovanni A. Botten, Hui Cao, Stuart H. Orkin, Michael Q. Zhang, Jian Xu
Format: Article
Language:English
Published: BMC 2020-03-01
Series:Genome Biology
Subjects:
CRISPR/Cas9
Chromatin
Epigenetics
3D genome
Enhancers
Online Access:http://link.springer.com/article/10.1186/s13059-020-01973-w

Internet

http://link.springer.com/article/10.1186/s13059-020-01973-w

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