Highly Efficient Transfer of Chromosomes to a Broad Range of Target Cells Using Chinese Hamster Ovary Cells Expressing Murine Leukemia Virus-Derived Envelope Proteins.

by Teruhiko Suzuki, Yasuhiro Kazuki, Mitsuo Oshimura, Takahiko Hara
Published 2016-01-01

A novel system for simultaneous or sequential integration of multiple gene-loading vectors into a defined site of a human artificial chromosome.

by Teruhiko Suzuki, Yasuhiro Kazuki, Mitsuo Oshimura, Takahiko Hara
Published 2014-01-01

Bioluminescence Measurement of Time-Dependent Dynamic Changes of CYP-Mediated Cytotoxicity in CYP-Expressing Luminescent HepG2 Cells

by Satoru Iwado, Satoshi Abe, Mitsuo Oshimura, Yasuhiro Kazuki, Yoshihiro Nakajima
Published 2021-03-01

A human artificial chromosome recapitulates the metabolism of native telomeres in mammalian cells.

by Michihito Wakai, Satoshi Abe, Yasuhiro Kazuki, Mitsuo Oshimura, Fuyuki Ishikawa
Published 2014-01-01

Development of Caco-2 cells expressing four CYPs via a mammalian artificial chromosome

by Yumi Ohta, Kanako Kazuki, Satoshi Abe, Mitsuo Oshimura, Kaoru Kobayashi, Yasuhiro Kazuki
Published 2020-08-01

A method for producing transgenic cells using a multi-integrase system on a human artificial chromosome vector.

by Shigeyuki Yamaguchi, Yasuhiro Kazuki, Yuji Nakayama, Eiji Nanba, Mitsuo Oshimura, Tetsuya Ohbayashi
Published 2011-01-01

Establishment and characterization of a transgenic mouse model for in vivo imaging of Bmp4 expression in the pancreas.

by Mayu Yasunaga, Nao Oumi, Mitsuhiko Osaki, Yasuhiro Kazuki, Tomoko Nakanishi, Mitsuo Oshimura, Kenzo Sato
Published 2011-01-01

Development of a multiple-gene-loading method by combining multi-integration system-equipped mouse artificial chromosome vector and CRISPR-Cas9.

by Kazuhisa Honma, Satoshi Abe, Takeshi Endo, Narumi Uno, Mitsuo Oshimura, Tetsuya Ohbayashi, Yasuhiro Kazuki
Published 2018-01-01

Establishment of a novel hepatocyte model that expresses four cytochrome P450 genes stably via mammalian-derived artificial chromosome for pharmacokinetics and toxicity studies.

by Daisuke Satoh, Satoru Iwado, Satoshi Abe, Kanako Kazuki, Shinobu Wakuri, Mitsuo Oshimura, Yasuhiro Kazuki
Published 2017-01-01

Pluripotent stem cell model of early hematopoiesis in Down syndrome reveals quantitative effects of short-form GATA1 protein on lineage specification.

by Shiori Matsuo, Yoko Nishinaka-Arai, Yasuhiro Kazuki, Mitsuo Oshimura, Tatsutoshi Nakahata, Akira Niwa, Megumu K Saito
Published 2021-01-01

Complete restoration of multiple dystrophin isoforms in genetically corrected Duchenne muscular dystrophy patient–derived cardiomyocytes

by Susi Zatti, Sebastian Martewicz, Elena Serena, Narumi Uno, Giovanni Giobbe, Yasuhiro Kazuki, Mitsuo Oshimura, Nicola Elvassore
Published 2014-01-01

Development of a Safeguard System Using an Episomal Mammalian Artificial Chromosome for Gene and Cell Therapy

by Narumi Uno, Katsuhiro Uno, Shinya Komoto, Teruhiko Suzuki, Masaharu Hiratsuka, Mitsuhiko Osaki, Yasuhiro Kazuki, Mitsuo Oshimura
Published 2015-01-01

Dual-color fluorescence imaging to monitor CYP3A4 and CYP3A7 expression in human hepatic carcinoma HepG2 and HepaRG cells.

by Saori Tsuji, Fumihiko Kawamura, Musashi Kubiura, Ayaka Hayashi, Tetsuya Ohbayashi, Yasuhiro Kazuki, Christophe Chesné, Mitsuo Oshimura, Masako Tada
Published 2014-01-01

Knockout of mouse Cyp3a gene enhances synthesis of cholesterol and bile acid in the liver

by Mari Hashimoto, Kaoru Kobayashi, Mio Watanabe, Yasuhiro Kazuki, Shoko Takehara, Asumi Inaba, Shin-ichiro Nitta, Naoto Senda, Mitsuo Oshimura, Kan Chiba
Published 2013-08-01

Use of a Human Artificial Chromosome for Delivering Trophic Factors in a Rodent Model of Amyotrophic Lateral Sclerosis

by Yasuhiro Watanabe, Yasuhiro Kazuki, Kanako Kazuki, Mitsutaka Ebiki, Mami Nakanishi, Kazuomi Nakamura, Miho Yoshida Yamakawa, Hiroyuki Hosokawa, Tetsuya Ohbayashi, Mitsuo Oshimura, Kenji Nakashima
Published 2015-01-01

N-Acetylcysteine prevents amyloid-β secretion in neurons derived from human pluripotent stem cells with trisomy 21

by Hiromitsu Toshikawa, Akihiro Ikenaka, Li Li, Yoko Nishinaka-Arai, Akira Niwa, Akira Ashida, Yasuhiro Kazuki, Tatsutoshi Nakahata, Hiroshi Tamai, David W. Russell, Megumu K. Saito
Published 2021-08-01

Transfer of a Mouse Artificial Chromosome into Spermatogonial Stem Cells Generates Transchromosomic Mice

by Takashi Shinohara, Kanako Kazuki, Narumi Ogonuki, Hiroko Morimoto, Shogo Matoba, Kei Hiramatsu, Kazuhisa Honma, Teruhiko Suzuki, Takahiko Hara, Atsuo Ogura, Mitsuo Oshimura, Mito Kanatsu-Shinohara, Yasuhiro Kazuki
Published 2017-10-01

Integration-free iPS cells engineered using human artificial chromosome vectors.

by Masaharu Hiratsuka, Narumi Uno, Kana Ueda, Hajime Kurosaki, Natsuko Imaoka, Kanako Kazuki, Etsuya Ueno, Yutaro Akakura, Motonobu Katoh, Mitsuhiko Osaki, Yasuhiro Kazuki, Masato Nakagawa, Shinya Yamanaka, Mitsuo Oshimura
Published 2011-01-01

Construction of stable mouse artificial chromosome from native mouse chromosome 10 for generation of transchromosomic mice

by Satoshi Abe, Kazuhisa Honma, Akane Okada, Kanako Kazuki, Hiroshi Tanaka, Takeshi Endo, Kayoko Morimoto, Takashi Moriwaki, Shusei Hamamichi, Yuji Nakayama, Teruhiko Suzuki, Shoko Takehara, Mitsuo Oshimura, Yasuhiro Kazuki
Published 2021-10-01

Blastocyst complementation using Prdm14-deficient rats enables efficient germline transmission and generation of functional mouse spermatids in rats

by Toshihiro Kobayashi, Teppei Goto, Mami Oikawa, Makoto Sanbo, Fumika Yoshida, Reiko Terada, Naoko Niizeki, Naoyo Kajitani, Kanako Kazuki, Yasuhiro Kazuki, Shinichi Hochi, Hiromitsu Nakauchi, M. Azim Surani, Masumi Hirabayashi
Published 2021-02-01