Alignment Computational Biology Data handling Data interpretation Efficient detection Enrichment algorithms Germ Cells Germline NGS data analysis High-Throughput Nucleotide Sequencing Humans Information analysis Interval padding Management challenges Mapping Next-generation sequencing Next-generation sequencing (NGS) Personnel testing Pipeline comparison Pipelines Polymorphism, Single Nucleotide Sequence alignments Short-read mappings Single nucleotides Software Variant calling biology germ cell high throughput sequencing human single nucleotide polymorphism