Alignment 1 Computational Biology 1 Data handling 1 Data interpretation 1 Efficient detection 1 Enrichment algorithms 1 Germ Cells 1 Germline NGS data analysis High-Throughput Nucleotide Sequencing 1 Humans 1 Information analysis 1 Interval padding 1 Management challenges 1 Mapping 1 Next-generation sequencing 1 Next-generation sequencing (NGS) 1 Personnel testing 1 Pipeline comparison 1 Pipelines 1 Polymorphism, Single Nucleotide 1 Sequence alignments 1 Short-read mappings 1 Single nucleotides 1 Software 1 Variant calling 1 biology 1 germ cell 1 high throughput sequencing 1 human 1 single nucleotide polymorphism 1