qPCR, dPCR, NGS – A journey

by Jim F. Huggett
Published 2015-03-01

RT-qPCR Diagnostics: The “Drosten” SARS-CoV-2 Assay Paradigm

by Stephen Bustin, Sara Kirvell, Jim F. Huggett, Tania Nolan
Published 2021-08-01

Methods for applying accurate digital PCR analysis on low copy DNA samples.

by Alexandra S Whale, Simon Cowen, Carole A Foy, Jim F Huggett
Published 2013-01-01

Evaluation of digital PCR for absolute RNA quantification.

by Rebecca Sanders, Deborah J Mason, Carole A Foy, Jim F Huggett
Published 2013-01-01

Making standards for quantitative real-time pneumococcal PCR

by Susan C. Morpeth, Jim F. Huggett, David R. Murdoch, J. Anthony G. Scott
Published 2014-12-01

Distribution of Tetrodotoxin in Pacific Oysters (<i>Crassostrea gigas</i>)

by Monika Dhanji-Rapkova, Andrew D. Turner, Craig Baker-Austin, Jim F. Huggett, Jennifer M. Ritchie
Published 2021-02-01

Assessing the Accuracy of Quantitative Molecular Microbial Profiling

by Denise M. O'Sullivan, Thomas Laver, Sasithon Temisak, Nicholas Redshaw, Kathryn A. Harris, Carole A. Foy, David J. Studholme, Jim F. Huggett
Published 2014-11-01

A novel approach for evaluating the performance of real time quantitative loop-mediated isothermal amplification-based methods

by Gavin J. Nixon, Helle F. Svenstrup, Carol E. Donald, Caroline Carder, Judith M. Stephenson, Stephen Morris-Jones, Jim F. Huggett, Carole A. Foy
Published 2014-12-01

Single base mutations in the nucleocapsid gene of SARS-CoV-2 affects amplification efficiency of sequence variants and may lead to assay failure

by Nathaniel Storey, Julianne R Brown, Rui P A Pereira, Denise M O'Sullivan, Jim F Huggett, Rachel Williams, Judith Breuer, Kathryn A Harris
Published 2021-09-01

Instability of 8E5 calibration standard revealed by digital PCR risks inaccurate quantification of HIV DNA in clinical samples by qPCR

by Eloise Busby, Alexandra S. Whale, R. Bridget Ferns, Paul R. Grant, Gary Morley, Jonathan Campbell, Carole A. Foy, Eleni Nastouli, Jim F. Huggett, Jeremy A. Garson
Published 2017-04-01

Integrating informatics tools and portable sequencing technology for rapid detection of resistance to anti-tuberculous drugs

by Jody E. Phelan, Denise M. O’Sullivan, Diana Machado, Jorge Ramos, Yaa E. A. Oppong, Susana Campino, Justin O’Grady, Ruth McNerney, Martin L. Hibberd, Miguel Viveiros, Jim F. Huggett, Taane G. Clark
Published 2019-06-01

Implications of storing urinary DNA from different populations for molecular analyses.

by Angela Cannas, Glendah Kalunga, Clare Green, Ludovica Calvo, Patrick Katemangwe, Klaus Reither, Mark D Perkins, Leonard Maboko, Michael Hoelscher, Elizabeth A Talbot, Peter Mwaba, Alimuddin I Zumla, Enrico Girardi, Jim F Huggett, TB trDNA consortium
Published 2009-09-01

Development of a highly sensitive liquid biopsy platform to detect clinically-relevant cancer mutations at low allele fractions in cell-free DNA.

by Davina Gale, Andrew R J Lawson, Karen Howarth, Mikidache Madi, Bradley Durham, Sarah Smalley, John Calaway, Shannon Blais, Greg Jones, James Clark, Peter Dimitrov, Michelle Pugh, Samuel Woodhouse, Michael Epstein, Ana Fernandez-Gonzalez, Alexandra S Whale, Jim F Huggett, Carole A Foy, Gerwyn M Jones, Hadas Raveh-Amit, Karin Schmitt, Alison Devonshire, Emma Green, Tim Forshew, Vincent Plagnol, Nitzan Rosenfeld
Published 2018-01-01

An inter-laboratory study to investigate the impact of the bioinformatics component on microbiome analysis using mock communities

by Denise M. O’Sullivan, Ronan M. Doyle, Sasithon Temisak, Nicholas Redshaw, Alexandra S. Whale, Grace Logan, Jiabin Huang, Nicole Fischer, Gregory C. A. Amos, Mark D. Preston, Julian R. Marchesi, Josef Wagner, Julian Parkhill, Yair Motro, Hubert Denise, Robert D. Finn, Kathryn A. Harris, Gemma L. Kay, Justin O’Grady, Emma Ransom-Jones, Huihai Wu, Emma Laing, David J. Studholme, Ernest Diez Benavente, Jody Phelan, Taane G. Clark, Jacob Moran-Gilad, Jim F. Huggett
Published 2021-05-01

An international comparability study on quantification of mRNA gene expression ratios: CCQM-P103.1

by Alison S. Devonshire, Rebecca Sanders, Alexandra S. Whale, Gavin J. Nixon, Simon Cowen, Stephen L.R. Ellison, Helen Parkes, P. Scott Pine, Marc Salit, Jennifer McDaniel, Sarah Munro, Steve Lund, Satoko Matsukura, Yuji Sekiguchi, Mamoru Kawaharasaki, José Mauro Granjeiro, Priscila Falagan-Lotsch, Antonio Marcos Saraiva, Paulo Couto, Inchul Yang, Hyerim Kwon, Sang-Ryoul Park, Tina Demšar, Jana Žel, Andrej Blejec, Mojca Milavec, Lianhua Dong, Ling Zhang, Zhiwei Sui, Jing Wang, Duangkamol Viroonudomphol, Chaiwat Prawettongsopon, Lina Partis, Anna Baoutina, Kerry Emslie, Akiko Takatsu, Sema Akyurek, Muslum Akgoz, Maxim Vonsky, L.A. Konopelko, Edna Matus Cundapi, Melina Pérez Urquiza, Jim F. Huggett, Carole A. Foy
Published 2016-06-01