Inventory of 'slow exchanging' hydrogen atoms in human proinsulin and its derivatives: observations on the mass spectrometric analysis of deuterio-proteins in D2O
Secondary structure elements of human proinsulin and of its tryptic products were compared by H/D exchange, in a single-pot, using mass spectrometry. Human proinsulin containing an N-terminal methionine, M-proinsulin, was engineered and converted into a perdeuterio derivative, which using an optimiz...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
2009-08.
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| Subjects: | |
| Online Access: | Get fulltext |