An in vitro transposon system for highly regulated gene expression: construction of Escherichia coli strains with arabinose-dependent growth at low temperatures
Placing a gene of interest under the control of an inducible promoter greatly aids the purification, localization and functional analysis of proteins but usually requires the sub-cloning of the gene of interest into an appropriate expression vector. Here, we describe an alternative approach employin...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
2001.
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| Subjects: | |
| Online Access: | Get fulltext |