Effect of DNase treatment on RNA extraction from preimplantation murine embryos

Effect of DNase treatment on RNA extraction from preimplantation murine embryos

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The quality of RNA is crucial when performing microarray experiments. This is particularly important when dealing with preimplantation embryos, from which a minimum yield of RNA of good quality can be produced. We report the optimization of several RNA extraction methods applied to preimplantation e...

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Bibliographic Details
Main Authors: Abdullah, B. (Author), Hoh, B.P (Author), Kadir, S.H.S.A (Author), Mohd-Fazirul, M. (Author), Nor-Ashikin, M.N.K (Author), Norhazlin, J. (Author), Norita, S. (Author), Rajikin, M.H (Author), Razif, D. (Author), Wan-Hafizah, W.J (Author)
Format: Article
Language:English
Published: Fundacao de Pesquisas Cientificas de Ribeirao Preto 2015
Subjects:
agar gel electrophoresis
animal
Animals
Article
Bioanalyzer
blastocyst
Blastocyst
controlled study
deoxyribonuclease
deoxyribonuclease I
Deoxyribonucleases
DNA microarray
DNase treatment
Electrophoresis, Agar Gel
embryo
female
Female
genetics
human
Humans
Institute for Cancer Research mouse
isolation and purification
messenger RNA
metabolism
Mice, Inbred ICR
Microarray
microarray analysis
morula
Morula
mouse
mouse embryo
nonhuman
nonparametric test
Oligonucleotide Array Sequence Analysis
preimplantation embryo
Preimplantation murine embryos
quality control
Quality Control
real time polymerase chain reaction
reverse transcription polymerase chain reaction
RNA
RNA extraction
RNA, Messenger
RT-qPCR
Statistics, Nonparametric
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