An alternate method for DNA and RNA extraction from clotted blood

An alternate method for DNA and RNA extraction from clotted blood

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We developed an alternative method to extract DNA and RNA from clotted blood for genomic and molecular investigations. A combination of the TRIzol method and the QIAamp spin column were used to extract RNA from frozen clotted blood. Clotted blood was sonicated and then the QIAamp DNA Blood Mini Kit...

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Bibliographic Details
Main Authors: Aziz, A.T.A (Author), Hoh, B.P (Author), Mokhtar, S.S (Author), Mokhtar, U. (Author), Umi, S.H (Author), Zaiharina, M.Z (Author), Zakaria, Z. (Author)
Format: Article
Language:English
Published: Fundacao de Pesquisas Cientificas de Ribeirao Preto 2013
Subjects:
article
blood
blood analysis
Blood Chemical Analysis
blood clot
chemistry
Clotted blood
copy number variation
DNA
DNA Copy Number Variations
DNA extraction
electrophoresis
Electrophoresis
gel electrophoresis
gene expression
genetic analysis
genetics
genomic DNA
genotype
Genotype
human
Humans
IL 6 gene
interleukin 6
isolation and purification
methodology
microarray analysis
polymerase chain reaction
Polymerase Chain Reaction
reverse transcription polymerase chain reaction
RNA
RNA extraction
Sonication
ultrasound
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