Detection of Four Porcine Enteric Coronaviruses Using CRISPR-Cas12a Combined with Multiplex Reverse Transcriptase Loop-Mediated Isothermal Amplification Assay

Detection of Four Porcine Enteric Coronaviruses Using CRISPR-Cas12a Combined with Multiplex Reverse Transcriptase Loop-Mediated Isothermal Amplification Assay

Porcine enteric coronaviruses have caused immense economic losses to the global pig industry, and pose a potential risk for cross-species transmission. The clinical symptoms of the porcine enteric coronaviruses (CoVs) are similar, making it difficult to distinguish between the specific pathogens by...

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Bibliographic Details
Main Authors:	Chen, X. (Author), Li, X. (Author), Liu, H. (Author), Liu, J. (Author), Liu, X. (Author), Niu, L. (Author), Shen, L. (Author), Tao, D. (Author), Xie, S. (Author), Xu, B. (Author), Zhao, S. (Author), Zhu, L. (Author)
Format:	Article
Language:	English
Published:	NLM (Medline) 2022
Subjects:	Alphacoronavirus animal Animals Coronavirinae Coronavirus Coronavirus infection Coronavirus Infections CRISPR Cas system CRISPR/Cas12a CRISPR-Cas Systems genetics molecular diagnosis Molecular Diagnostic Techniques multiplex loop-mediated isothermal amplification nucleic acid amplification techniques Nucleic Acid Amplification Techniques pig porcine enteric coronaviruses Porcine epidemic diarrhea virus RNA directed DNA polymerase RNA-Directed DNA Polymerase sensitivity and specificity Sensitivity and Specificity single-strand DNA-FQ reporter Swine swine disease Swine Diseases veterinary medicine
Online Access:	View Fulltext in Publisher

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