Detection of Four Porcine Enteric Coronaviruses Using CRISPR-Cas12a Combined with Multiplex Reverse Transcriptase Loop-Mediated Isothermal Amplification Assay

Detection of Four Porcine Enteric Coronaviruses Using CRISPR-Cas12a Combined with Multiplex Reverse Transcriptase Loop-Mediated Isothermal Amplification Assay

Porcine enteric coronaviruses have caused immense economic losses to the global pig industry, and pose a potential risk for cross-species transmission. The clinical symptoms of the porcine enteric coronaviruses (CoVs) are similar, making it difficult to distinguish between the specific pathogens by...

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Bibliographic Details
Main Authors: Chen, X. (Author), Li, X. (Author), Liu, H. (Author), Liu, J. (Author), Liu, X. (Author), Niu, L. (Author), Shen, L. (Author), Tao, D. (Author), Xie, S. (Author), Xu, B. (Author), Zhao, S. (Author), Zhu, L. (Author)
Format: Article
Language:English
Published: NLM (Medline) 2022
Subjects:
Alphacoronavirus
animal
Animals
Coronavirinae
Coronavirus
Coronavirus infection
Coronavirus Infections
CRISPR Cas system
CRISPR/Cas12a
CRISPR-Cas Systems
genetics
molecular diagnosis
Molecular Diagnostic Techniques
multiplex loop-mediated isothermal amplification
nucleic acid amplification techniques
Nucleic Acid Amplification Techniques
pig
porcine enteric coronaviruses
Porcine epidemic diarrhea virus
RNA directed DNA polymerase
RNA-Directed DNA Polymerase
sensitivity and specificity
Sensitivity and Specificity
single-strand DNA-FQ reporter
Swine
swine disease
Swine Diseases
veterinary medicine
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