Establishment of CRISPR/Cas9 Genome-Editing System Based on Dual sgRNAs in Flammulina filiformis

Establishment of CRISPR/Cas9 Genome-Editing System Based on Dual sgRNAs in Flammulina filiformis

Flammulina filiformis, previously known as Asian Flammulina velutipes, is one of the most commercially important edible fungi, with nutritional value and medicinal properties worldwide. However, precision genome editing using CRISPR/Cas9, which is a revolutionary technology and provides a powerful t...

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Bibliographic Details
Main Authors: Dong, J. (Author), Ge, F. (Author), Jiang, A. (Author), Liao, J. (Author), Liu, X. (Author), Qiu, H. (Author), Ren, A. (Author), Shi, L. (Author), Tian, L. (Author), Wu, T. (Author), Yu, H. (Author), Zhao, M. (Author), Zhu, J. (Author)
Format: Article
Language:English
Published: MDPI 2022
Subjects:
CRISPR/Cas9
dual sgRNAs
Flammulina filiformis
gene editing
pyrG
U6 promoter
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