Establishment of an Epicutaneously Sensitized Murine Model of Shellfish Allergy and Evaluation of Skin Condition by Raman Microscopy

• Main Authors: Endo, A. (Author), Ichimura-Shimizu, M. (Author), Ishimaru, S. (Author), Kakimoto, T. (Author), Kobayashi, T. (Author), Kojima, T. (Author), Matsumoto, M. (Author), Minamikawa, T. (Author), Miyakami, Y. (Author), Ogawa, H. (Author), Oya, T. (Author), Sumida, S. (Author), Tsunematsu, T. (Author), Tsuneyama, K. (Author), Wai, C.Y.Y (Author)
• Format: Article
• Language: English
• Published: MDPI 2022
• Subjects: epicutaneous sensitization; food allergy; Raman microscopy; shellfish; shrimp; skin; tape-strip; tropomyosin
• Online Access: View Fulltext in Publisher
• ISBN: 20763417 (ISSN)
• DOI: 10.3390/app12073566

Background: Shellfish allergy is one of the most common food allergies. Recent studies have shown that sensitization to allergens via the skin is involved in the development of food allergies. In this study, a mouse model of shrimp allergy was generated by epicutaneous sensitization and used to identify skin conditions associated with susceptibility to sensitization. Methods: Four-week-old female BALB/c mice were sensitized by repeated application of 0.1 mg of tropomyosin to tapestripped skin on days 0, 7, and 15, followed by a challenge on days 28 and 35. Results: Epicutaneously sensitized mice exhibited higher serum levels of tropomyosin-specific IgE on day 15 than control mice. After the oral challenge, model mice had higher anaphylaxis scores and lower rectal temperature. After three tape-strip treatments for sensitization, the skin was analyzed by Raman microscopy. The sensitized mice exhibited lower relative intensities of Raman bands at 399, 915, and 1073 cm−1 than control mice, which could be helpful noninvasive markers in screening for potential sensitization via the skin. Conclusions: An epicutaneous sensitization shellfish allergy model was generated. This model will be useful in studies to elucidate the pathogenesis of skin sensitization. Raman microscopy may also be valuable for capturing subtle skin changes leading to sensitization. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.