Generation of 4-vinylguaiacol through a novel high-affinity ferulic acid decarboxylase to obtain smoke flavours without carcinogenic contaminants

Traditional smoke flavours bear the risk of containing a multitude of contaminating carcinogenic side-products. Enzymatic decarboxylation of ferulic acid released from agro-industrial side-streams by ferulic acid esterases (FAE) enables the sustainable generation of pure, food grade 4-vinylguaiacol...

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Bibliographic Details
Main Authors:	Berger, R.G (Author), Detering, T. (Author), Mundry, K. (Author)
Format:	Article
Language:	English
Published:	Public Library of Science 2021
Subjects:	4 vinylguaiacol 4-vinylguaiacol affinity chromatography amino acid sequence amino terminal sequence Article beta sheet budding yeast caffeic acid carboxylesterase Carboxylic Ester Hydrolases carboxylyase Carboxy-Lyases carcinogen Carcinogens chemistry cinnamic acid complementary DNA controlled study Cordyceps coumaric acid Coumaric Acids decarboxylation enzyme active site enzyme activity enzyme phosphorylation enzyme purification enzyme stability FAD gene ferulic acid ferulic acid decarboxylase flavor flavoring agent Flavoring Agents gene gene amplification gene expression green chemistry guaiacol Guaiacol hydrogen bond hydrophilicity isolation and purification Komagataella Komagataella phaffii metabolism Michaelis Menten kinetics molecular cloning nickel complex nonhuman phenylacrylic acid decarboxylase RNA isolation Saccharomycetales Sanger sequencing Schiff base Schizophyllum Schizophyllum commune sequence alignment sinapic acid smoke synthesis unclassified drug
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Generation of 4-vinylguaiacol through a novel high-affinity ferulic acid decarboxylase to obtain smoke flavours without carcinogenic contaminants

Internet

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