Standard protocol for the PIGRET assay, a high-throughput reticulocyte Pig-a assay with an immunomagnetic separation, used in the interlaboratory trial organized by the Mammalian Mutagenicity Study Group of the Japanese Environmental Mutagen and Genome Society

The PIGRET assay is one of the Pig-a assays targeting reticulocytes (RETs), an in vivo genotoxicity evaluation method using flow cytometry with endogenous reporter glycosylphosphatidylinositol anchor protein. The PIGRET assay with RETs selectively enriched with anti-CD71 antibodies has several desir...

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Bibliographic Details
Main Authors: Chikura, S. (Author), Horibata, K. (Author), Itoh, S. (Author), Kimoto, T. (Author), Muto, S. (Author), Sanada, H. (Author)
Format: Article
Language:English
Published: BioMed Central Ltd 2021
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