Kinetic modeling of phosphorylase-catalyzed iterative β-1,4-glycosylation for degree of polymerization-controlled synthesis of soluble cello-oligosaccharides

Kinetic modeling of phosphorylase-catalyzed iterative β-1,4-glycosylation for degree of polymerization-controlled synthesis of soluble cello-oligosaccharides

Background: Cellodextrin phosphorylase (CdP; EC 2.4.1.49) catalyzes the iterative β-1,4-glycosylation of cellobiose using α-d-glucose 1-phosphate as the donor substrate. Cello-oligosaccharides (COS) with a degree of polymerization (DP) of up to 6 are soluble while those of larger DP self-assemble in...

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Bibliographic Details
Main Authors: Klimacek, M. (Author), Nidetzky, B. (Author), Zhong, C. (Author)
Format: Article
Language:English
Published: BioMed Central Ltd 2021
Subjects:
Biocatalytic synthesis
Bottom-up oligosaccharide synthesis
Cadmium compounds
carbohydrate
catalysis
Catalysis
Catalysts
Cellodextrin phosphorylase
Cellodextrin phosphorylase (EC 2.4.1.49)
Cello-oligosaccharides
cellulose
Cellulose
Clostridium cellulosi
Complex relationships
Curricula
Degree of polymerization
Enzymatic glycosylation
enzyme activity
Glucose
Glycosylation
Iterative glycosylation
Kinetic analysis and modeling
Kinetic theory
Kinetics
numerical model
Oligosaccharides
Parameter determination
Phosphorylation
polymerization
Polymerization
reaction kinetics
Substrate concentrations
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