To denoise or to cluster, that is not the question: optimizing pipelines for COI metabarcoding and metaphylogeography

To denoise or to cluster, that is not the question: optimizing pipelines for COI metabarcoding and metaphylogeography

Background: The recent blooming of metabarcoding applications to biodiversity studies comes with some relevant methodological debates. One such issue concerns the treatment of reads by denoising or by clustering methods, which have been wrongly presented as alternatives. It has also been suggested t...

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Bibliographic Details
Main Authors: Antich, A. (Author), Palacin, C. (Author), Turon, X. (Author), Wangensteen, O.S (Author)
Format: Article
Language:English
Published: BioMed Central Ltd 2021
Subjects:
adenosine deaminase
Adenosine Deaminase
biodiversity
Biodiversity
cluster analysis
Cluster Analysis
Clustering
Clustering algorithms
Clustering methods
COI
Cytochrome oxidase I
Denoising
DNA barcoding
DNA Barcoding, Taxonomic
Intercellular Signaling Peptides and Proteins
Intra-cluster
Marine community
Metabarcoding
Metaphylogeography
Natural variability
Operational taxonomic units
Parameter setting
Pipelines
Protein-coding genes
Sequence variants
signal peptide
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