|
|
|
|
| LEADER |
04119nam a2200889Ia 4500 |
| 001 |
10.1016-j.jpha.2020.03.010 |
| 008 |
220427s2021 CNT 000 0 und d |
| 020 |
|
|
|a 20951779 (ISSN)
|
| 245 |
1 |
0 |
|a Comprehensive metabolic profiling of Alismatis Rhizoma triterpenes in rats based on characteristic ions and a triterpene database
|
| 260 |
|
0 |
|b Xi'an Jiaotong University
|c 2021
|
| 856 |
|
|
|z View Fulltext in Publisher
|u https://doi.org/10.1016/j.jpha.2020.03.010
|
| 520 |
3 |
|
|a Alismatis Rhizoma (AR) is widely used in Chinese medicine, and its major bioactive components, triterpenes, reportedly possess various pharmacological activities. Therefore, it is very important to study the metabolism of triterpenes in vivo. However, the metabolism of AR triterpene extract has not been comprehensively elucidated due to its complex chemical components and metabolic pathways. In this study, an ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry method, which was based on the characteristic ions from an established database of known triterpenes, was used to analyze the major metabolites in rats following the oral administration of Alismatis Rhizoma extracts (ARE). As a result, a total of 233 constituents, with 85 prototype compounds and 148 metabolites, were identified for the first time. Hydrogenation, oxidation, sulfate and glucuronidation conjugation were the major metabolic pathways for triterpenes in AR. In addition, the mutual in vivo transformation of known ARE triterpenes was discovered and confirmed for the first time. Those results provide comprehensive insights into the metabolism of AR in vivo, which will be useful for future studies on its pharmacodynamics and pharmacokinetics. Moreover, this established strategy may be useful in metabolic studies of similar compounds. © 2020 Xi'an Jiaotong University
|
| 650 |
0 |
4 |
|a 11 dehydro 22 hydroxy 24,25 dideoxy alisol a
|
| 650 |
0 |
4 |
|a 11 dehydro 24 deoxy alisol a
|
| 650 |
0 |
4 |
|a 11 dehydro 25 deoxy alisol a
|
| 650 |
0 |
4 |
|a 11,24 dideoxy 16 oxo alisol a
|
| 650 |
0 |
4 |
|a 11,24,25 trideoxy 22 hydroxy 16 oxo alisol a
|
| 650 |
0 |
4 |
|a 11,25 dideoxy 16 oxo alisol a
|
| 650 |
0 |
4 |
|a 13,17 epoxy alisol b
|
| 650 |
0 |
4 |
|a 16 oxo alisol a 24 acetate
|
| 650 |
0 |
4 |
|a 22 hydroxy 16,23 oxido alisol b
|
| 650 |
0 |
4 |
|a 22 hydroxyalisol b
|
| 650 |
0 |
4 |
|a 22 oxo 24 deoxy alisol a
|
| 650 |
0 |
4 |
|a 22 oxo 25 deoxy alisol a
|
| 650 |
0 |
4 |
|a 23 dehydro 16 oxo alisol a
|
| 650 |
0 |
4 |
|a 24 oxo alisol a
|
| 650 |
0 |
4 |
|a Alismatis extract
|
| 650 |
0 |
4 |
|a Alismatis rhizoma
|
| 650 |
0 |
4 |
|a alisol b 23 acetate
|
| 650 |
0 |
4 |
|a alisol c 23 acetate
|
| 650 |
0 |
4 |
|a alisol f
|
| 650 |
0 |
4 |
|a animal experiment
|
| 650 |
0 |
4 |
|a Article
|
| 650 |
0 |
4 |
|a bile
|
| 650 |
0 |
4 |
|a blood analysis
|
| 650 |
0 |
4 |
|a chemical analysis
|
| 650 |
0 |
4 |
|a conjugation
|
| 650 |
0 |
4 |
|a controlled study
|
| 650 |
0 |
4 |
|a data base
|
| 650 |
0 |
4 |
|a dehydrogenation
|
| 650 |
0 |
4 |
|a drug identification
|
| 650 |
0 |
4 |
|a drug metabolite
|
| 650 |
0 |
4 |
|a drug transformation
|
| 650 |
0 |
4 |
|a feces analysis
|
| 650 |
0 |
4 |
|a glucuronidation
|
| 650 |
0 |
4 |
|a HPLC-QTOF-MS/MS
|
| 650 |
0 |
4 |
|a hydrogenation
|
| 650 |
0 |
4 |
|a in vivo study
|
| 650 |
0 |
4 |
|a ion
|
| 650 |
0 |
4 |
|a male
|
| 650 |
0 |
4 |
|a metabolic fingerprinting
|
| 650 |
0 |
4 |
|a Metabolites
|
| 650 |
0 |
4 |
|a nonhuman
|
| 650 |
0 |
4 |
|a oxidation kinetics
|
| 650 |
0 |
4 |
|a pharmacodynamics
|
| 650 |
0 |
4 |
|a pharmacokinetic parameters
|
| 650 |
0 |
4 |
|a quadrupole mass spectrometry
|
| 650 |
0 |
4 |
|a rat
|
| 650 |
0 |
4 |
|a sulfate
|
| 650 |
0 |
4 |
|a sulfuric acid
|
| 650 |
0 |
4 |
|a tandem mass spectrometry
|
| 650 |
0 |
4 |
|a tifenamil
|
| 650 |
0 |
4 |
|a time of flight mass spectrometry
|
| 650 |
0 |
4 |
|a triterpene derivative
|
| 650 |
0 |
4 |
|a Triterpenes
|
| 650 |
0 |
4 |
|a ultra performance liquid chromatography
|
| 650 |
0 |
4 |
|a unclassified drug
|
| 650 |
0 |
4 |
|a urinalysis
|
| 700 |
1 |
|
|a Cheng, Z.
|e author
|
| 700 |
1 |
|
|a Du, Z.
|e author
|
| 700 |
1 |
|
|a Feng, Y.
|e author
|
| 700 |
1 |
|
|a Jiang, H.
|e author
|
| 700 |
1 |
|
|a Li, J.
|e author
|
| 700 |
1 |
|
|a Li, S.
|e author
|
| 700 |
1 |
|
|a Ouyang, H.
|e author
|
| 700 |
1 |
|
|a Wang, L.
|e author
|
| 773 |
|
|
|t Journal of Pharmaceutical Analysis
|
