Colorado potato beetle alpha-amylase: Purification, action pattern and subsite mapping for exploration of active centre

Colorado potato beetle alpha-amylase: Purification, action pattern and subsite mapping for exploration of active centre

Colorado potato beetle is an invasive insect herbivore and one of the most challenging agricultural pests globally. This study is the first characterization of the active centre of Colorado potato beetle (Leptinotarsa decemlineata) α-amylase (LdAmy). Bond cleavage frequency values for LdAmy were det...

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Bibliographic Details
Main Authors: Gyémánt, G. (Author), Hámori, C. (Author), Kandra, L. (Author), Remenyik, J. (Author)
Format: Article
Language:English
Published: Elsevier B.V. 2021
Subjects:
affinity chromatography
alpha-Amylases
amino acid sequence
Amino Acid Sequence
amylase
animal
Animals
Article
beetle
binding affinity
binding site
Binding Sites
Bond cleavage frequency
catalysis
Catalytic Domain
Coleoptera
Colorado potato beetle
controlled study
enzyme active site
enzyme analysis
enzyme purification
enzyme specificity
enzymology
genetics
high performance liquid chromatography
human
Humans
hydrolysis
Hydrolysis
Insect α-amylase
isolation and purification
metabolism
nonhuman
pig
protein binding
Protein Binding
protein cleavage
protein purification
sequence alignment
sequence homology
Sequence Homology, Amino Acid
Subsite structure
Substrate Specificity
Swine
Tenebrio
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