Modified Cross-Linking, Ligation, and Sequencing of Hybrids (qCLASH) to Identify MicroRNA Targets

Modified Cross-Linking, Ligation, and Sequencing of Hybrids (qCLASH) to Identify MicroRNA Targets

This protocol was designed to identify microRNA (miRNA) targetomes from smaller-input samples by performing a simplified workflow of the Cross-Linking and Sequencing of Hybrids (CLASH) technique developed in the Tollervey group. In this ribonomics-based technique, Cross-Linking and Immunoprecipitati...

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Bibliographic Details
Main Authors: Gay, L.A (Author), Renne, R. (Author), Turner, P.C (Author)
Format: Article
Language:English
Published: John Wiley and Sons Inc 2021
Subjects:
Argonaute
argonaute protein
Argonaute Proteins
Article
breast cancer cell line
cancer tissue
cell count
cell culture
DNA cross linking
Endothelial Cells
endothelium cell
enzyme mechanism
enzyme purification
Epstein Barr virus
Epstein Barr virus infection
Epstein-Barr Virus Infections
gene loss
genetics
Herpesvirus 4, Human
human
human cell
Human herpesvirus 8
human tissue
Humans
immunoprecipitation
ligation
metastatic melanoma
microRNA
MicroRNA
MicroRNAs
qCLASH
ribonomics
RNA analysis
RNA ligase
RNA replication
RNA sequencing
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