Nanopore ReCappable sequencing maps SARS-CoV-2 5′ capping sites and provides new insights into the structure of sgRNAs

The SARS-CoV-2 virus has a complex transcriptome characterised by multiple, nested subgenomic RNAsused to express structural and accessory proteins. Long-read sequencing technologies such as nanopore direct RNA sequencing can recover full-length transcripts, greatly simplifying the assembly of struc...

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Main Authors: Akeson, M. (Author), Almuqrin, A. (Author), Birney, E. (Author), Castelli, M. (Author), Clementi, M. (Author), Clementi, N. (Author), Corrêa, I.R., Jr (Author), Criscuolo, E. (Author), Davidson, A.D (Author), Ettwiller, L. (Author), Frigè, G. (Author), Giambruno, R. (Author), Jain, M. (Author), Leger, A. (Author), Leonardi, T. (Author), Mancini, N. (Author), Matthews, D.A (Author), Mulroney, L. (Author), Nicassio, F. (Author), Olsen, H. (Author), Schildkraut, I. (Author), Tzertzinis, G. (Author), Ugolini, C. (Author), Williamson, M.K (Author), Wulf, M.G (Author)
Format: Article
Language:English
Published: Oxford University Press 2022
Online Access:View Fulltext in Publisher