Spacer2PAM: A computational framework to guide experimental determination of functional CRISPR-Cas system PAM sequences
RNA-guided nucleases from CRISPR-Cas systems expand opportunities for precise, targeted genome modification. Endogenous CRISPR-Cas systems in many prokaryotes are attractive to circumvent expression, functionality, and unintended activity hurdles posed by heterologous CRISPR-Cas effectors. However,...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Oxford University Press
2022
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| Online Access: | View Fulltext in Publisher |