Protocol for in vitro lysine deacetylation to test putative substrates of class III deacetylases

Lysine acetylation is an important post-translational modification that is used in multiple cellular pathways, such as the regulation of gene expression at the histone level. The purpose of this assay is to test for putative substrates of class III deacetylases using an in vitro method. The in vitro...

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Bibliographic Details
Main Authors: Minten, E.V (Author), Yu, D.S (Author)
Format: Article
Language:English
Published: Cell Press 2022
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Online Access:View Fulltext in Publisher