Optimization of in vitro transcription/translation conditions for in vitro compartmentalization studies and synthesis of 4-fluorohistidine
Genetic code expansion allows the incorporation of non-canonical amino acids with a variety of new functional groups: fluorescent amino acids,1-3 azides,4-6 alkynes,5-10 and photocrosslinkers.4,11,12 This incorporation requires the evolution of new tRNA/aminoacyl tRNA sythetase pairs. Traditiona...
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| Format: | Others |
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VCU Scholars Compass
2017
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| Online Access: | http://scholarscompass.vcu.edu/etd/4807 http://scholarscompass.vcu.edu/cgi/viewcontent.cgi?article=5877&context=etd |