Targeted Gene Editing Using CRISPR/Cas9 in a Wheat Protoplast System

The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system has become a promising tool for targeted gene editing in a variety of organisms including plants. In this system, a 20 nt sequence on a single guide RNA (sgRNA) is the only gene-specific information required to modify...

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Bibliographic Details
Main Author: Cui, Xiucheng
Other Authors: Ouellet, Thérèse
Language:en
Published: Université d'Ottawa / University of Ottawa 2017
Subjects:
Online Access:http://hdl.handle.net/10393/36543
http://dx.doi.org/10.20381/ruor-20823