Engineering pH tolerant mutants of a cyanide dihydratase of Bacillus pumilus C1 and identifying constraints on substrate specificity in nitrilases

Engineering pH tolerant mutants of a cyanide dihydratase of Bacillus pumilus C1 and identifying constraints on substrate specificity in nitrilases

This study generated two cyanide dihydratase (CynD) mutants of Bacillus pumilus C1 with improved activity at higher pH by random mutagenesis. The purpose of this study was to create enzyme variants better suited to degrade cyanide under the harsh conditions of industrial applications. We employed er...

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Bibliographic Details
Main Author: Wang, Lan
Other Authors: Benedik, Michael J.
Format: Others
Language:en_US
Published: 2010
Subjects:
protein engineering
cyanide remediation
bioremediation
cyanide dihydratase
nitrilase
microbiology
gene cloning
genetic engineer
Online Access:http://hdl.handle.net/1969.1/ETD-TAMU-2765
http://hdl.handle.net/1969.1/ETD-TAMU-2765

Internet

http://hdl.handle.net/1969.1/ETD-TAMU-2765
http://hdl.handle.net/1969.1/ETD-TAMU-2765

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