Příprava rekombinantní cDNA některých lékových transportérů

Příprava rekombinantní cDNA některých lékových transportérů

CDS for ABCC4 and ABCC5 transporters were amplified. For ABCC5 two systems were outlined, however the only first one was used. The optimal annealing temperature was 64 řC; optimal concentration of magnesium was 2mM. TOPO XL have proved best results in our research in between cloning kits we tried. T...

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Main Author: Svobodová, Iveta
Other Authors: Štaud, František
Format: Dissertation
Language:Czech
Published: 2008
Online Access:http://www.nusl.cz/ntk/nusl-292535
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spelling ndltd-nusl.cz-oai-invenio.nusl.cz-2925352017-09-02T04:15:51Z Příprava rekombinantní cDNA některých lékových transportérů Prepsration of recombinant cDNA of drug transporters Svobodová, Iveta Štaud, František Červený, Lukáš CDS for ABCC4 and ABCC5 transporters were amplified. For ABCC5 two systems were outlined, however the only first one was used. The optimal annealing temperature was 64 řC; optimal concentration of magnesium was 2mM. TOPO XL have proved best results in our research in between cloning kits we tried. The CDS for ABCC4 and ABCC5 were cloned into the vector. Inserts were rended with the use of restrictive endonucleasis. ABCC4 was gashed by Spe I and Not I and ABCC5 by EcoR I and Hind III. With the same endonucleasis, the process of rending was repeated for pZeoSV2(-). Afterwards, the inserts were cloned into pZeoSV2(-). 2008 info:eu-repo/semantics/masterThesis http://www.nusl.cz/ntk/nusl-292535 cze info:eu-repo/semantics/restrictedAccess
collection NDLTD
language Czech
format Dissertation
sources NDLTD
description CDS for ABCC4 and ABCC5 transporters were amplified. For ABCC5 two systems were outlined, however the only first one was used. The optimal annealing temperature was 64 řC; optimal concentration of magnesium was 2mM. TOPO XL have proved best results in our research in between cloning kits we tried. The CDS for ABCC4 and ABCC5 were cloned into the vector. Inserts were rended with the use of restrictive endonucleasis. ABCC4 was gashed by Spe I and Not I and ABCC5 by EcoR I and Hind III. With the same endonucleasis, the process of rending was repeated for pZeoSV2(-). Afterwards, the inserts were cloned into pZeoSV2(-).
author2 Štaud, František
author_facet Štaud, František
Svobodová, Iveta
author Svobodová, Iveta
spellingShingle Svobodová, Iveta
Příprava rekombinantní cDNA některých lékových transportérů
author_sort Svobodová, Iveta
title Příprava rekombinantní cDNA některých lékových transportérů
title_short Příprava rekombinantní cDNA některých lékových transportérů
title_full Příprava rekombinantní cDNA některých lékových transportérů
title_fullStr Příprava rekombinantní cDNA některých lékových transportérů
title_full_unstemmed Příprava rekombinantní cDNA některých lékových transportérů
title_sort příprava rekombinantní cdna některých lékových transportérů
publishDate 2008
url http://www.nusl.cz/ntk/nusl-292535
work_keys_str_mv AT svobodovaiveta pripravarekombinantnicdnanekterychlekovychtransporteru
AT svobodovaiveta prepsrationofrecombinantcdnaofdrugtransporters
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