Příprava rekombinantní cDNA některých lékových transportérů
CDS for ABCC4 and ABCC5 transporters were amplified. For ABCC5 two systems were outlined, however the only first one was used. The optimal annealing temperature was 64 řC; optimal concentration of magnesium was 2mM. TOPO XL have proved best results in our research in between cloning kits we tried. T...
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| Format: | Dissertation |
| Language: | Czech |
| Published: |
2008
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| Online Access: | http://www.nusl.cz/ntk/nusl-292535 |