Development and optimization of a diagnostic system based on Illumina sequencing of genus wide PCR amplicons for the detection of viruses of grapevines
In this study we present a poly-specific PCR-based, high-throughput sequencing (HTS) diagnostic system together with an appropriate data analysis pipeline for the diagnosis of grapevine viruses. Poly-specific and virus-specific primers were established to be capable of detecting and identifying 3...
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| Language: | en |
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University of Pretoria
2017
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| Online Access: | http://hdl.handle.net/2263/63362 Wayland, J 2017, Development and optimization of a diagnostic system based on Illumina sequencing of genus wide PCR amplicons for the detection of viruses of grapevines, MSc Dissertation, University of Pretoria, Pretoria, viewed yymmdd <http://hdl.handle.net/2263/63362> |
Internet
http://hdl.handle.net/2263/63362Wayland, J 2017, Development and optimization of a diagnostic system based on Illumina sequencing of genus wide PCR amplicons for the detection of viruses of grapevines, MSc Dissertation, University of Pretoria, Pretoria, viewed yymmdd <http://hdl.handle.net/2263/63362>