An Analysis of Efficiency and Melt Curve Effects on Quantitative Polymerase Chain Reaction (qPCR) Inhibition

An Analysis of Efficiency and Melt Curve Effects on Quantitative Polymerase Chain Reaction (qPCR) Inhibition

Real-time or quantitative PCR (qPCR) is an innovative method used to determine the amount of amplifiable DNA in a biological sample. Typically, a fluorescent dye is introduced during thermal cycling, causing a change in fluorescent output as the double-stranded DNA (dsDNA) product accumulates. Both...

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Bibliographic Details
Main Author: Thompson, Robyn E
Format: Others
Published: FIU Digital Commons 2010
Subjects:
qPCR
Inhibition
DNA
Binding
Melt
Amplification
Efficiency
Forensic
Online Access:http://digitalcommons.fiu.edu/etd/329
http://digitalcommons.fiu.edu/cgi/viewcontent.cgi?article=1408&context=etd

Internet

http://digitalcommons.fiu.edu/etd/329
http://digitalcommons.fiu.edu/cgi/viewcontent.cgi?article=1408&context=etd

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