Development of a synthetic biology approach to targeted directed evolution of proteins in vivo
This thesis describes the development of a novel platform for targeted directed evolution, designed to operate entirely in vivo. The system comprises a fusion of T7 RNA polymerase and activation-induced deaminase (AID); targeting is achieved by the placement of the sequence of interest under the con...
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Imperial College London
2013
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| Online Access: | http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.676762 |