In vitro reconstitution of the extraordinary post-translational processing of Concanavalin A precursor : circular sequence permutation by enzymatic cleavages and protein splicing
The lectin Concanavalin A is processed <i>in planta </i>by a splicing-mediated circular permutation of its initial single-chain precursor, pro-Concanavalin A. Active (carbohydrate-binding) protein conformations can be purified by dextran-affinity chromatography. This thesis demonstrates...
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Swansea University
2003
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| Online Access: | http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.637901 |