Protein damage during purification : understanding the effects of size exclusion chromatography on the structure of biosynthetic human insulin (BHI)
Purification of Biosynthetic Human Insulin (BHI or Humulin), involves multimodal chromatographies as well as intermediate crystallizations. Size exclusion chromatography (SEC) is the final chromatographic step in BHI purification, which is conducted in acetic acid at high protein concentrations. The...
| Main Author: | |
|---|---|
| Published: |
University College London (University of London)
2011
|
| Subjects: | |
| Online Access: | http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.625655 |