Rapid microscale evaluation of the impact of fermentation conditions on inclusion body formation, solubilisation and protein refolding yields
Heterologous protein expression in E. coli can lead to the formation of dense insoluble aggregates named inclusion bodies (IB). The refolding of protein derived from IB is often characterised by low yields of active product. Process optimisation is often achieved empirically and requires significant...
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University College London (University of London)
2013
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| Online Access: | http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.617910 |